The field of proteomics aims to characterise the complete repertoire of proteins expressed by a genome (the proteome) in order to better understand how cells function. The global analysis of proteins is challenging due to the high complexity (>12,000 genes are frequently transcriptionally active) and dynamic range of protein abundances. (10 orders of magnitude between high- and low-expressed proteins). A further challenge is the post-translational modifications of proteins (e.g. phosphorylation) and their interactions with one another to form complexes, both of which are highly divergent in time and space. To tackle these analytical challenges, proteomics employs a combination of techniques including sophisticated sample preparation methods, mass spectrometry (MS) and bioinformatics.
THE CNIO PROTEOMICS UNIT
The main mission of CNIO Proteomics Unit is to provide expert support and advice to CNIO research groups in research projects related to proteomic technologies, as well as to implement and develop state-of-the-art tools and workflows for cancer research. The three research topics of the Unit are proteome expression profiling, post-translational modifications and protein-protein interactions. The driving force of our work is the implementation of diverse quantitative mass spectrometry-based proteomics approaches.
The Proteomics Unit belongs to the CNIO Biotechnology Programme which is currently composed of nine Core Units covering major areas in Proteomics, Genomics , Monoclonal Antibodies, Histopathology, Flow Cytometry, Confocal Microscopy, Molecular Imaging and Transgenic Mice, as well as an Animal Facility. Although the Core Units are mainly focused on meeting the internal demand generated by the CNIO research groups, they also provide services and collaborate with groups from other institutions, both public and private.
The CNIO Proteomics Unit is also part of the ProteoRed-ISCIII (www.proteored.org) which is a National Network for the coordination, integration and development of the Spanish Proteomics Facilities providing proteomics services for supporting Spanish researchers in the field of proteomics.